About the Talk
Presenter
Özge Gizlenci
Özge Gizlenci received her BSc in Molecular Biology and Genetics from Middle East Technical University in Türkiye. She then went on to an MSc in Molecular Biosciences at Heidelberg University, specialising in Cancer Biology. In October 2018 she started a PhD position in the Immunology Programme of the Babraham Institute, funded by a Marie Skłodowska-Curie Action under the COSMIC consortium of the European Union’s Horizon 2020 research and innovation programme. Her PhD project with Dr. Martin Turner focuses on understanding changes in gene expression and alternative splicing in B cells in the germinal centre, using long-read next-generation sequencing technologies.
Abstract
Alternative splicing (AS) plays an important role in the differentiation of immune cells during the immune response, as 29% of AS genes are immune-specific. To understand post-transcriptional regulation in human germinal centre B cells at both the gene and the isoform level, we set out to develop a long-read Oxford Nanopore Technologies (ONT) workflow. We developed a workflow called ‘Nexons’ to identify different splicing variants. An in-depth analysis of splicing regulators with Nexons revealed that poison exons of splicing factors such as SRSF3 are preferentially removed upon activation.
Date: May 20th, 2022 – 10:30 AM (GMT+3)
Language: English